Journal:

Article Title: Expression of prostanoid receptors in human ductus arteriosus

doi: 10.1038/sj.bjp.0705092

Figure Lengend Snippet: Primer design for PCR amplification of prostanoid receptors and β-actin

Article Snippet: Abundance of receptor mRNA was expressed relative to β-actin mRNA. table ft1 table-wrap mode="anchored" t5 caption a7 Primer design for PCR amplification of prostanoid receptors and β -actin Open in a separate window

Techniques: Amplification

Journal:

Article Title: Expression of prostanoid receptors in human ductus arteriosus

doi: 10.1038/sj.bjp.0705092

Figure Lengend Snippet: Expression of prostanoid receptor mRNA in human DA. RNA was isolated from excised tissue, reverse-transcribed by oligo(dT) and PCR amplification for all tissue samples was repeated three times. A fragment of β-actin was amplified as internal control. A representative expression pattern of one tissue sample is shown, base pair markers (bp) are indicated on both sides.

Article Snippet: Abundance of receptor mRNA was expressed relative to β-actin mRNA. table ft1 table-wrap mode="anchored" t5 caption a7 Primer design for PCR amplification of prostanoid receptors and β -actin Open in a separate window

Techniques: Expressing, Isolation, Reverse Transcription, Amplification, Control

Journal:

Article Title: Expression of prostanoid receptors in human ductus arteriosus

doi: 10.1038/sj.bjp.0705092

Figure Lengend Snippet: Expression of the different prostanoid receptors relative to the expression of β-actin. RNA was isolated from excised tissue, reverse-transcribed by oligo(dT) and PCR amplification was performed for the indicated prostanoid receptors. Intensity of DNA bands is presented as percentage of amplification of β-actin fragment.

Article Snippet: Abundance of receptor mRNA was expressed relative to β-actin mRNA. table ft1 table-wrap mode="anchored" t5 caption a7 Primer design for PCR amplification of prostanoid receptors and β -actin Open in a separate window

Techniques: Expressing, Isolation, Reverse Transcription, Amplification

Journal: Microorganisms

Article Title: Functional Analysis of Conserved Hypothetical Proteins from the Antarctic Bacterium, Pedobacter cryoconitis Strain BG5 Reveals Protein Cold Adaptation and Thermal Tolerance Strategies

doi: 10.3390/microorganisms10081654

Figure Lengend Snippet: Primer sequences for qPCR analysis of P. cryoconitis genes.

Article Snippet: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms10081654/s1 , Figure S1: DNA amplification of pcbg5hp1 , pcbg5hp2 , and pcbg5hp12 from P. cryoconitis BG5; Table S1: Primer design for PCR amplification of selected P. cryoconitis genes; Table S2: Gene’s identification information of P. cryoconitis conserved HPs related to thermal stress response; Figure S2: Agarose gel electrophoresis analysis of total RNA samples extracted from P. cryoconitis ; Figure S3: SDS-PAGE analysis of the purified protein of PCBG5HP1, PCBG5HP2, and PCBG5HP12 proteins from P. cryoconitis BG5.

Techniques: Sequencing, Amplification

Journal: Microorganisms

Article Title: Functional Analysis of Conserved Hypothetical Proteins from the Antarctic Bacterium, Pedobacter cryoconitis Strain BG5 Reveals Protein Cold Adaptation and Thermal Tolerance Strategies

doi: 10.3390/microorganisms10081654

Figure Lengend Snippet: The physicochemical properties of P. cryoconitis HPs retrieved by the Protparam tool.

Article Snippet: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms10081654/s1 , Figure S1: DNA amplification of pcbg5hp1 , pcbg5hp2 , and pcbg5hp12 from P. cryoconitis BG5; Table S1: Primer design for PCR amplification of selected P. cryoconitis genes; Table S2: Gene’s identification information of P. cryoconitis conserved HPs related to thermal stress response; Figure S2: Agarose gel electrophoresis analysis of total RNA samples extracted from P. cryoconitis ; Figure S3: SDS-PAGE analysis of the purified protein of PCBG5HP1, PCBG5HP2, and PCBG5HP12 proteins from P. cryoconitis BG5.

Techniques: Molecular Weight

Journal: Microorganisms

Article Title: Functional Analysis of Conserved Hypothetical Proteins from the Antarctic Bacterium, Pedobacter cryoconitis Strain BG5 Reveals Protein Cold Adaptation and Thermal Tolerance Strategies

doi: 10.3390/microorganisms10081654

Figure Lengend Snippet: The BLAST search results for the selected HPs from P. cryoconitis against the NCBI non-redundant UniProtKB/SwissProt sequences database.

Article Snippet: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms10081654/s1 , Figure S1: DNA amplification of pcbg5hp1 , pcbg5hp2 , and pcbg5hp12 from P. cryoconitis BG5; Table S1: Primer design for PCR amplification of selected P. cryoconitis genes; Table S2: Gene’s identification information of P. cryoconitis conserved HPs related to thermal stress response; Figure S2: Agarose gel electrophoresis analysis of total RNA samples extracted from P. cryoconitis ; Figure S3: SDS-PAGE analysis of the purified protein of PCBG5HP1, PCBG5HP2, and PCBG5HP12 proteins from P. cryoconitis BG5.

Techniques: Sequencing

Journal: Microorganisms

Article Title: Functional Analysis of Conserved Hypothetical Proteins from the Antarctic Bacterium, Pedobacter cryoconitis Strain BG5 Reveals Protein Cold Adaptation and Thermal Tolerance Strategies

doi: 10.3390/microorganisms10081654

Figure Lengend Snippet: Functional annotation of P. cryoconitis HPs by the InterProScan tool.

Article Snippet: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms10081654/s1 , Figure S1: DNA amplification of pcbg5hp1 , pcbg5hp2 , and pcbg5hp12 from P. cryoconitis BG5; Table S1: Primer design for PCR amplification of selected P. cryoconitis genes; Table S2: Gene’s identification information of P. cryoconitis conserved HPs related to thermal stress response; Figure S2: Agarose gel electrophoresis analysis of total RNA samples extracted from P. cryoconitis ; Figure S3: SDS-PAGE analysis of the purified protein of PCBG5HP1, PCBG5HP2, and PCBG5HP12 proteins from P. cryoconitis BG5.

Techniques: Functional Assay, Binding Assay, Activity Assay

Journal: Microorganisms

Article Title: Functional Analysis of Conserved Hypothetical Proteins from the Antarctic Bacterium, Pedobacter cryoconitis Strain BG5 Reveals Protein Cold Adaptation and Thermal Tolerance Strategies

doi: 10.3390/microorganisms10081654

Figure Lengend Snippet: Heat-induced denaturation of citrate synthase. A total of 300 nM recombinant proteins of P. cryoconitis BG5 were purified and incubated at 43 °C with 150 nM citrate synthase, and turbidity was measured at 320 nm. Data were representative of three biological replicates and were expressed as the mean ± SD. BSA at 300 nM was a control sample for citrate synthase non-specific protection.

Article Snippet: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/microorganisms10081654/s1 , Figure S1: DNA amplification of pcbg5hp1 , pcbg5hp2 , and pcbg5hp12 from P. cryoconitis BG5; Table S1: Primer design for PCR amplification of selected P. cryoconitis genes; Table S2: Gene’s identification information of P. cryoconitis conserved HPs related to thermal stress response; Figure S2: Agarose gel electrophoresis analysis of total RNA samples extracted from P. cryoconitis ; Figure S3: SDS-PAGE analysis of the purified protein of PCBG5HP1, PCBG5HP2, and PCBG5HP12 proteins from P. cryoconitis BG5.

Techniques: Recombinant, Purification, Incubation, Control